Figure 8.

Cross-reactivity of anti-GD2 antibodies with gangliosides GM1, GM2, GD1b and GD3. (A, B) Interaction of anti-GD2 antibodies with gangliosides GD2 vs. GM1, GM2, GD1b and GD3 was assessed by ELISA as described in Methods. Plates were coated with gangliosides GD2, GM1, GM2, GD1b, and GD3 (0.25 μg/well) and incubated with two types of anti-GD2 mAbs (0.156 - 10 μg/ml) ME361 (A) and 14G2a (B). In (A), the level of cross-reactivity is presented as the ratio for GM2, GD1b and GD3 binding to that of GD2. Mean ± S.E. of three separate experiments are shown, statistical analysis was performed using two-way analysis of variance method. There was a statistically significant difference between groups (P ≤ 0.001), ***P <0.001 as determined by multiple comparisons versus zero cross-reactivity by Dunnett's post-hoc analysis (A). In (B), multiple comparisons were not performed, since the values of the cross-reactivity were less than 2%.