Table 1.
Differentially expressed protein spots between X. citri and hrpB − strains statically cultured in XVM2 with a change abundance of minimum 1.5 fold and p value of < 0.05 (ANOVA)
| X. citri gene no. | Protein name | MOWSE score | Accession no. | Predicted MW/p I | Observed MW/p I | Peptide match/coverage | Fold change compared to WT |
|---|---|---|---|---|---|---|---|
| 01 Metabolism | |||||||
| 01.01 Amino acid metabolism | |||||||
| XAC0125 |
Aspartate/tyrosine/aromatic aminotransferase |
350 |
Q8PR41_XANAC |
43.3/5.72 |
49.0/4.8 |
19/38% |
1.9 |
| XAC4034 |
Shikimate 5-dehydrogenase |
297 |
AROE_XANAC |
29.9/4.93 |
30.0/5.9 |
19/17% |
2.4 |
| XAC2717 |
Tryptophan synthase subunit b |
31 |
TRPB_XANAC |
43.3/5.88 |
53.0/4.6 |
2/4% |
7.5 |
| XAC3709 |
Tryptophan repressor binding protein |
48 |
Q8PGA8_XANAC |
20.0/6.40 |
10.0/4.4 |
3/17% |
−1.6 |
| 01.02 Nitrogen, sulfur and selenium metabolism | |||||||
| XAC0554 |
NAD(PH) nitroreductase |
208 |
Y554_XANAC |
21.0/5.83 |
18.0/4.7 |
14/38% |
4.6 |
| 01.03 Nucleotide/nucleoside/nucleobase metabolism | |||||||
| XAC1716 |
CTP-synthase |
125 |
PYRG_XANAC |
61.7/5.91 |
67.0/4.5 |
14/21% |
3.5 |
| 01.05 C-compounds and carbohydrate metabolism | |||||||
| XAC2077 |
Succinate dehydrogenase flavoprotein subunit |
192 |
Q8PKT5_XANAC |
65.8/5.89 |
66.0/4.6 |
20/25% |
2.2 |
| XAC1006 |
Malate dehydrogenase |
1054 |
MDH_XANAC |
34.9/5.37 |
45.0/5.4 |
55/50% |
−1.8 |
| XAC3579 |
Phosphohexose mutases (XanA) |
98 |
Q8PGN7_XANAC |
49.1/5.29 |
54.0/5.6 |
7/10% |
1.7 |
| XAC3585 |
DTP-glucose 4,6-dehydratase |
235 |
Q8PGN1_XANAC |
38.6/5.86 |
48.0/4.7 |
12/17% |
2.1 |
| XAC0612 |
Cellulase |
245 |
Q8PPS3_XANAC |
51.6/5.76 |
57.0/4.9 |
23/32% |
2.6 |
| XAC3225 |
Transglycosylase |
178 |
Q8PHM6_XANAC |
46.2/5.89 |
53.0/4.8 |
14/22% |
−1.6 |
| 01.06 Lipid, fatty acid and isoprenoid metabolism | |||||||
| XAC3300 |
Putative esterase precursor (EstA) |
96 |
Q8PHF7_XANAC |
35.9/6.03 |
62.0/6.2 |
3/4% |
−3.1 |
| XAC1484 |
Short chain dehydrogenase precursor |
104 |
Q8PME5_XANAC |
26.0/5.97 |
30.0/4.4 |
5/9% |
2.2 |
| 01.06.02 Membrane lipid metabolism | |||||||
| XAC0019 |
Outer membrane protein (FadL) |
167 |
Q8PRE4_XANAC |
47.3/5.18 |
46.0/6.1 |
8/10% |
−10.0 |
| XAC0019 |
Outer membrane protein (FadL) |
79 |
Q8PRE4_XANAC |
47.3/5.18 |
35.0/6.0 |
7/13% |
−6.2 |
| 01.20 Secondary metabolism | |||||||
| XAC4109 |
Coproporphyrinogen III oxidase |
46 |
HEM6_XANAC |
34.6/5.81 |
37.0/4.9 |
8/19% |
1.5 |
| 02 Energy | |||||||
| 02.01 Glycolysis and gluconeogenesis | |||||||
| XAC1719 |
Enolase |
90 |
ENO_XANAC |
46.0/4.93 |
55.0/5.9 |
7/13% |
1.7 |
| XAC3352 |
Glyceraldehyde-3-phosphate dehydrogenase |
267 |
Q8PHA7_XANAC |
36.2/6.03 |
46.0/4.4 |
24/28% |
2.6 |
| XAC2292 |
UTP-glucose-1-phosphate uridylyltransferase (GalU) |
92 |
Q8PK83_XANAC |
32.3/5.45 |
38.0/5.3 |
13/30% |
4.2 |
| 02.07 Pentose phosphate pathway | |||||||
| XAC3372 |
Transketolase 1 |
85 |
Q8PH87_XANAC |
72.7/5.64 |
69.0/4.9 |
5/7% |
5.0 |
| 02.11 Electron transport and membrane-associated energy conservation | |||||||
| XAC3587 |
Electron transfer flavoprotein a subunit |
50 |
Q8PGM9_XANAC |
31.8/4.90 |
34.0/5.5 |
6/14% |
2.3 |
| 10 Cell cycle and DNA processing | |||||||
| 10.03 Cell cycle |
|
|
|||||
| XAC1224 |
Cell division topological specificity factor (MinE) |
33 |
MINE_XANAC |
9.6/5.37 |
12.0/4.9 |
1/14% |
2.7 |
| 10.03.03 Cytokinesis/septum formation and hydrolysis | |||||||
| XAC1225 |
Septum site-determining protein (MinD) |
143 |
Q8PN48_XANAC |
28.9/5.32 |
34.0/5.6 |
19/26% |
2.3 |
| 11 Transcription | |||||||
| XAC0996 |
DNA-directed RNA polymerase subunit a |
104 |
RPOA_XANAC |
36.3/5.58 |
33.0/5.0 |
5/7% |
−4.3 |
| XAC0966 |
DNA-directed RNA polymerase subunit b |
150 |
RPOC_XANAC |
155.7/7.82 |
35.0/4.6 |
16/8% |
−3.3 |
| 14 Protein fate (folding, modification and destination) | |||||||
| 14.01 Protein folding and stabilization | |||||||
| XAC0542 |
60 kDa chaperonin (GroEL) |
199 |
CH60_XANAC |
57.1/5.05 |
41.0/5.5 |
15/27% |
−11.2 |
| 16 Protein with binding function or cofactor requirement | |||||||
| XAC2726 |
Adenine-specific methylase (Dam methylase) |
84 |
Q8PJ19_XANAC |
33.9/4.70 |
41.0/6.2 |
10/12% |
−1.9 |
| XAC1362 |
GTN reductase |
44 |
Q8PMR4_XANAC |
39.4/5.37 |
50.0/5.3 |
7/10% |
2.3 |
| XAC3664 |
OmpW family outer membrane protein precursor |
226 |
Q8PN48_XANAC |
23.8/4.97 |
28.0/6.2 |
12/13% |
2.3 |
| 30 Cellular communication/Signal transduction mechanism | |||||||
| XAC0291 |
Oar protein ( TonB-dependent transporter) |
50 |
Q8PQN2_XANAC |
107.9/5.29 |
108.0/5.7 |
2/1% |
4.3 |
| XAC2672 |
Oar protein ( TonB-dependent transporter) |
280 |
Q8PJ70_XANAC |
117.4/5.10 |
90.0/5.9 |
19/18% |
2.4 |
| XAC4273 |
TonB-dependent transporter |
100 |
Q8PJL0_XANAC |
109.2/5.14 |
90.0/5.6 |
3/3% |
2.8 |
| XAC1143 |
TonB-dependent transporter |
576 |
Q8PND0_XANAC |
87.7/5.21 |
70.0/6.1 |
30/33% |
1.7 |
| XAC3050 |
TonB-dependent transporter |
596 |
Q8PI48_XANAC |
105.8/4.76 |
64.0/6.2 |
30/16% |
−3.0 |
| XAC3444 |
TonB-dependent transporter |
1280 |
Q8PH16_XANAC |
103.2/4.79 |
90.0/6.3 |
84/37% |
3.9 |
| XAC3168* |
TonB-dependent transporter |
98 |
Q8PHT1_XANAC |
87.3/5.20 |
59.0/6.0 |
3/3% |
−3.1 |
| XAC3166* |
TonB-dependent transporter |
410 |
Q8PHT3_XANAC |
84.5/4.95 |
69.0/6.1 |
22/18% |
−2.9 |
| XAC3489 |
TonB-dependent transporter |
685 |
Q8PGX3_XANAC |
88.9/4.93 |
69.0/5.9 |
40/24% |
−1.7 |
| XAC1413 |
Outer membrane protein assembly factor BamA |
135 |
Q8PML3_XANAC |
87.6/5.53 |
88.0/5.4 |
13/15% |
2.8 |
| 32 Cell rescue, defense and virulence | |||||||
| XAC2504* |
Regulator of pathogenicity factors (RpfN) |
271 |
Q8PJM6_XANAC |
41.3/5.98 |
49.0/4.4 |
21/16% |
−4.8 |
| XAC0907 |
Alkyl hydroperoxide reductase subunit C |
240 |
O06464_XANAC |
20.6/6.15 |
20.0/4.2 |
28/61% |
1.3 |
| 32.07 Cellular detoxification | |||||||
| XAC1474 |
Glutathione transferase |
39 |
Q8PMF5_XANAC |
23.9/6.06 |
22.0/4.7 |
4/8% |
1.7 |
| 34 Interaction with the environment |
|
|
|
|
|
|
|
| 34.01 Homeostasis |
|
|
|
|
|
|
|
| XAC1149 |
Bacterioferritin |
100 |
Q8PNC4_XANAC |
21.2/4.71 |
20.0/6.3 |
6/20% |
2.1 |
| XAC0493 |
Bacterioferritin |
152 |
Q8PQ38_XANAC |
18.3/4.80 |
12.0/6.5 |
19/43% |
2.5 |
| XAC1533 |
Dihydrolipoamide dehydrogenase |
336 |
Q8PM99_XANAC |
50.5/5.80 |
59.0/4.6 |
34/47% |
4.0 |
| 42 Biogenesis of cellular components |
|
|
|
|
|
|
|
| XAC1230 |
Putative membrane protein |
71 |
Q8PN43_XANAC |
43.1/6.88 |
24.0/4.4 |
4/11% |
−3.5 |
| 99 Unclassified proteins |
|
|
|
|
|
|
|
| XAC1262 |
Protein of unknown function (Aminopeptidase) |
121 |
Q8PN12_XANAC |
63.4/5.85 |
68.0/4.6 |
13/15% |
5.3 |
| XAC1344 | Protein of unknown function (CcmA) | 67 | Q8PMT2_XANAC | 18.7/5.45 | 23.0/5.7 | 4/18% | −1.7 |
*Protein spots 240 and 398 were previously named “ferric enterobactin receptor” are now classified as TonB-dependent transporter, while protein spot 31 previously identified as “carbohydrate selective porin” and is now classified as Regulator of pathogenicity factors.