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Analysis of 2′-SCF3 modified RNA: anion-exchange HPLC traces (top) of 14 nt RNA (A) and 32 nt RNA (B), and respective LC-ESI mass spectra (bottom). HPLC conditions: Dionex DNAPac column (4 × 250 mm), 80 °C, 1 mL min–1, 0 to 60% buffer B in 45 min. Buffer A: Tris-HCl (25 mM), urea (6 M), pH 8.0. Buffer B: Tris-HCl (25 mM), urea (6 M), NaClO4 (0.5 M), pH 8.0. For LC-ESI MS conditions, see the Supporting Information.
