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Thermal stabilities of unmodified and 2′-SCF3 modified oligoribonucleotides. UV-melting profiles of (A) self-complementary 8 nt RNA, (B) 15 nt RNA hairpin, (C) 17 nt RNA hairpin, and (D) 14 nt RNA duplex. Conditions: cRNA = 8 μM for profiles A and B, 4 μM for profiles C and D; 10 mM Na2HPO4, 150 mM NaCl, pH 7.0. Nucleotide abbreviations in red indcate the 2′-SCF3 modified position.
