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. 2014 Apr 25;136(18):6656–6663. doi: 10.1021/ja5005637

Figure 3.

Figure 3

ECOSY NMR spectrum of the single-stranded RNA 5′-GU(2′-SCF3-U)CG. For the 2′-SCF3 uridine moiety, the 3-bond scalar coupling constant of H1′ and H2′ (3JH1′-H2′) was extracted from the corresponding crosspeak and amounted to 10.4 Hz. Assuming a pure C2′/C3′-endo equilibrium, this value is correlated to a C2′-endo (South) population of 100%.29,30 For the other single-stranded RNA nucleotides, coupling constants of 8.5 to 9.0 Hz were measured corresponding to C2′-endo populations between 84 to 89%. Conditions: cRNA = 0.3 mM; 25 mM sodium cacodylate, pH 7.0, 298 K.