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. 2014 Apr 29;7:7. doi: 10.1186/1755-1536-7-7

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Copyright © 2014 Prasad et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic diagram depicting epithelial-primary fibroblast co-culture scratch wound model. (A) A549 and fibroblasts are attached on either side of trans-well insert coated with collagen. (B) Scratch wound is created by running a sterile pipette tip across. (C) Resultant denuded area. (D) Fibroblast transmigration across trans-well into denuded area.