Table 4.
Strains and plasmids used in this study
| Strains or plasmids | Genotype/description | Sources |
|---|---|---|
| Strains | ||
|
E. coli BL21 star(DE3) |
F−ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) |
Invitrogen |
|
E. coli BL21 star(DE3) ΔfadD |
Knockout of fadD encoding acyl-CoA synthetase |
This study |
| Plasmids | ||
| pACYCduet-1 |
Cm
r
oriP15A lacI
q
T7p |
Novagen |
| pET30a |
Kan
r
oripBR322 lacI
q
T7p |
Novagen |
| pEASY-Blunt |
Kan
r
Amp
r
oripUC |
Transgen |
| pE-ssi2 |
pET30a harboring Arabidopsis fatty-acid desaturase |
[59] |
| pA-acc |
pACYCduet-1 harboring E. coli acetyl-CoA carboxylase |
[60] |
| PEASY-AtFatA |
pEASY-Blunt harboring Arabidopsis thioesterase |
This study |
| pA-AtFatA |
pACYCduet-1 harboring Arabidopsis thioesterase |
This study |
| pE-AtFatAssi2 |
pET30a harboring Arabidopsis fatty-acid desaturase and thioesterase |
This study |
| pKD46 |
AproriR101 repA(Ts) λ Red (γ, β and exo) |
Coli Genetic Stock Center |
| pKD4 |
FRT-Kan
r
-FRT oriR6K |
Coli Genetic Stock Center |
| pCP20 | AprCmrrepA(Ts) FLP | Coli Genetic Stock Center |