Table 1.
Geometric parameters of guanine crystals in Phelsuma species
| Species (individual) |
Green/blue |
White |
Red |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Orientation A/y 0 (FWHM) a | Crystal height, nm b | Crystal spacing, nm b | Orientation A/y 0 (FWHM) | Crystal height, nm | Crystal length, nm | Orientation A/y 0 (FWHM) | Crystal height, nm | Crystal length, nm | |
|
P. grandis (1) |
15.0 (32°) |
80.8 ± 12.2/ 78.5 ± 10.9c |
96.0 ± 20.8/ 104.7 ± 25.5c |
2.7 (72°) |
84 ± 44 |
173 ± 84 |
1.3 (82°) |
68 ± 34 |
128 ± 70 |
|
P. grandis (2) |
9.7 (49°) |
81.0 ± 12.9 |
84.9 ± 22.7 |
2.0 (61°) |
78 ± 39 |
169 ± 96 |
1.1 (53°) |
67 ± 33 |
124 ± 82 |
|
P. grandis (3) |
13.0 (47°) |
79.4 ± 10.7 |
99.2 ± 10.6 |
2.6 (59°) |
75 ± 36 |
171 ± 91 |
1.7 (46°) |
71 ± 37 |
130 ± 73 |
|
P. laticauda |
13.3 (39°) |
82.5 ± 11.4 |
97.3 ± 12.6 |
2.2 (60°) |
71 ± 41 |
194 ± 140 |
2.6 (61°) |
75 ± 42 |
154 ± 87 |
|
P. quadriocellata |
20.3 (40°) |
70.0 ± 22.7 |
37.9 ± 13.8 |
6.4 (49°) |
84 ± 42 |
209 ± 126 |
4.8 (67°) |
69 ± 41 |
171 ± 103 |
|
P. klemmeri |
9.6 (32°) |
68.0 ± 10.8 |
93.2 ± 26.3 |
4.5 (58°) |
72 ± 47 |
265 ± 209 |
NAd |
NAd |
NAd |
| P. lineata | 6.9 (42°) | 74.0 ± 11.1 | 109.6 ± 21.8 | 4.8 (50°) | 94 ± 57 | 226 ± 137 | 2.1 (58°) | 68 ± 43 | 170 ± 107 |
aThe ratio A/y0 and the full-width half maximum (FWHM) of the Gaussian curve describe the crystal orientation relative to the skin surface (see Methods).
bMean ± standard deviation are given for crystal height and spacing between layers of well-organized crystals in green and blue skin, and for crystal height and length in white and red skin. Note that the parameters describing crystal orientation differ substantially between blue/green and white/red skin.
cThe two numbers shown for crystal height and spacing of green/blue skin in P. grandis individual 1 correspond to samples taken from the back and the neck (blue and yellowish-green, respectively; see Figure 4b). Note that the spacing between crystal layers is substantially larger in the neck sample. Additional data are provided for blue and green skin samples, including a comparison of the simulated colors to the real colors seen in the skin after pigment removal (see Additional file 2: Table S3).
dNA, data not available because of the absence of red skin in P. klemmeri.