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Quantification of representative miRNAs of Ascaris lumbricoides and A. suum. Three novel miRNAs of each nematode were detected. Two miRNAs named asu-miR-novel-031 and alu-miR-novel-53 had significant expression differences of 5p and 3p mature miRNAs. The alu-miR-novel-64-5p and asu-miR-novel-383-3p were not successfully amplified, which might due to their low expression levels.
