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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Mar 13;1843(6):1216–1224. doi: 10.1016/j.bbamcr.2014.03.005

Figure 3.

Figure 3

Effect of TMEFF2 in different ECM substrates. A) TMEFF2 significantly inhibits cell spreading on vitronectin-coated surfaces. RWPE2 cells expressing FL_TMEFF2, TMEFF2_ΔGA, or the vector were plated onto cover glass coated with the different ECM substrates. Percentages of spread cells were analyzed 3 h after seeding. Data are presented as mean ± SD of five independent experiments. B) TMEFF2 inhibits RWPE2 cell migration towards vitronectin but not fibronectin as measured in a Boyden chamber assay. Shown are representative images of RWPE2 cells expressing the different TMEFF2 constructs after 48 h migration (top), and quantification of those images by densitometric analysis using ImageJ (bottom). Data are presented as mean ± SD of three independent experiments.