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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Mar 13;1843(6):1216–1224. doi: 10.1016/j.bbamcr.2014.03.005

Figure 5.

Figure 5

TMEFF2 inhibits Rho activation. RWPE2 cells expressing FL_TMEFF2, TMEFF2_ΔGA, or the vector were incubated in serum-free EpiLife CF/PRF medium for 3 days and then stimulated with 10% FBS for 2 min. Levels of GTP-bound RhoA were determined by G-LISA RhoA activation assays. Data are presented as mean ± SD of five independent experiments (top). Rhodamine phalloidin staining of the cells stimulated with 10% FBS for 10 min (bottom) demonstrating lack of stress fibers in the cells expressing FL_TMEFF2. Scale bar, 25 μm.