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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Semin Cell Dev Biol. 2009 Aug 12;20(8):931–941. doi: 10.1016/j.semcdb.2009.08.005

Figure 3.

Figure 3

Fibrillar collagen density of the chick embryo CAM. A chicken embryo (at day 12) was injected i.v. with rhodamin-conjugated lens culinaris agglutinin (LCA), and the CAM was harvested after 20 min, fixed in Zn-formalin, and analyzed by MP microscopy. Collagen fibres as detected by SHG (green), ecto/ endodermal layer of epithelial cells (white arrowheads) and blood cells (located capillary plexus or extravasated; empty arrowheads) were detected by autofluorescence signal (blue-gray); blood vessels positive for rhodamin-LCA fluorescence (red, asterisks). Image processing was performed as described in Fig. 2. Bars, 100 μm.