Figure 3.
(A) Western blots for G6PD of LNCaP-neo and LNCaP-p52 cells. Tubulin was used as a loading control. (B) NADPH/NADP ratio of LNCaP-p52 cells compared to LNCaP-neo cells. (C) Transient transfection of p52 enhances glucose metabolism in CWR22Rv1 cells. Immunoblots of HA tagged p52, PKM2 and tubulin in transient transfected LNCaP cells (left). Glucose consumption assay in transient vehicle and p52 transfected CWR22Rv1 cells (right). Data was presented as mean ±SD of three independent experiments. *, p < 0.05, **, p<0.01.