Developmental changes in ascorbate (a), free GSH (b), and α-tocopherol (c) concentrations in the brain regions of postnatal day (P) 7, P14 and P60 rats. Values are mean ± SD, n=6-7. There is a main effect of postnatal age on all three antioxidants in all the brain regions (p<0.001 for each, ANOVA). The concentration of each compound differs between P7, P14 and P60 in all the brain regions (p<0.01, Bonferroni-adjusted unpaired t tests) except, there are no difference in GSH concentrations between P7 and P14 in ctx, hip, str, crb, hyp and mid, and between P7 and P60 in olf, and no difference in α-tocopherol concentrations between P7 and P14 in olf and str, and between P7 and P60 in hyp. Abbreviations: olf, olfactory bulb; ctx, cerebral cortex; hip, hippocampus; str, striatum; hyp, hypothalamus; mid, midbrain; crb, cerebellum; pns, pons; mob, medulla oblongata; chol, cholesterol; and GSH, glutathione.