Fig. 1.

Protocols used for differentiation of adipocyte-derived stem cells into insulin-producing cells. Human or mouse adipose tissue-derived stem cells (ADSCs) and human eyelid adipose tissue-derived stem cells (HEACs) were used for differentiation. The factors used for differentiation at each stage, and in vitro characterization and outcomes of in vivo functional tests of the differentiated insulin-producing cells are denoted. DMEM: dulbecco’s modified eagle’ medium, HGF: hepatocyte growth factor, FBS: fetal bovine serum, GLP-1: glucagon-like peptide, BSA: bovine serum albumin, ITS: insulin-transferrin-selenium, Sod. butyrate: sodium butyrate, 2-ME: 2-mercaptoethanol, NEAA: non essential amino acids