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. 2008 Nov;1(1):70–81. doi: 10.15283/ijsc.2008.1.1.70

Fig. 1.

Fig. 1.

Flow cytometric histogram from CD34+ and CD34– cells isolated from cultured human hair follicle cells. Cells were stained with fluorescein isothiocyanate-conjugated antibodies against CD34, CD45 and phycoerythrin-conjugated antibodies against CD133, CD24, CD29, CD44 or immunoglobulin isotype control antibodies. Cells were analyzed using fluorescence-activated cell sorter Calibur. FSC/SSC gating to exclude debris. (A) The percentages of isolated CD34+ cells expressing FITC or PE are given. Staining on FACS analysis with CD34 was greater positive than 98%, and CD133, CD44, CD24, CD29 was also positive, whereas CD45was negative. (B) The percentages of isolated CD34− cells expressing FITC or PE are given. Staining on FACS analysis with CD34 was negative and CD45, CD133 was also negative, whereas CD24, CD29, CD44 was strongly positive, same as CD34+ population.