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. 2009 May;2(1):51–58. doi: 10.15283/ijsc.2009.2.1.51

Table 1.

Sequence of primers used for RT-PCR and length of fragments

Genes Sequences Tm (°C) Size (bp)
Nanog F: 5′-TTC TTG ACT GGG ACC TTG TC-3′ 54 300
R: 5′-GCT TGC CTT GCT TTG AAG CA-3′
NF-68 F: 5′-GAG TGA AAT GGC ACG ATA CCT A-3′ 58 500
R: 5′-TTT CCT CTC CTT CTT CTT CAC CTT C-3′
α-CA F: 5′-GGA GTT ATG GTG GGT ATG GGT C-3′ 58 500
R: 5′-AGT GGT GAC AAA GGA GTA GCC A-3′
hAFP F: 5′-AGC TTG GTG GAT GAA AC-3′ 50 200
R: 5′-TCC AAC AGG CCT GAG AAA TC-3′
TERT F: 5′-GAG CTG ACG TGG AAG ATG AG-3′ 55 300
R: 5′-CTT CAA GTG CTG TCT GAT TCC AAT G-3′
OC F: 5′-ATG AGA GCC CTC ACA CTC CTC-3′ 62 293
R: 5′-GCC GTA GAA GCG CCG ATA GGC-3′
Col I F: 5′-CAT CTC AGA AGC AGA ATC TCC-3′ 59 360
R: 5′-CCA TAA ACC ACA CTA TCA CCT C-3′
Run×2 F: 5′-CCG CAC GAC AAC GCG ACC AT-3′ 55 288
R: 5′-CGC TCC GGC CCA CAA ATC TC-3′
β-actin F: 5′-TCC TTC TGC ATC CTG TCA GCA-3′ 58 300
R: 5′-CAG GAG ATG GCC ACT GCC GCA-3′