Fig. 4.

Proliferative potential of hESCs maintained in hypoxia (5% O2) in the presence of inhibitors of Notch and Hh signaling. The hESC line CLS1 was treated with 1 μM γ-SI, 10 μM CP or their combination for 3 weeks. (A) Representative image demonstrating incorporation of BrdU. The scale bars indicate 1 mm. (B) [³H]-thymidine incorporation. The values are presented as the mean and the error bars denote the standard error of mean. (C) Western blot analysis of NF-κB. Beta-actin was visualized for the purpose of normalization and the plotted data were normalized to the control. ^*Indicate statistical significance (p<0.05) with respect to the control, ^†Indicates statistical significance (p<0.05) of the combined treatment compared with single inhibitors.