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List of primers for real-time RT-PCR
| Gene | Upstream primer | Downstream primer |
|---|---|---|
| Oct4 | CTGGTTCGCTTTCTC | GGGGGTTCTATTTGG |
| Nanog | AGGAAGAGTAGAGGC | CAACTGGCCGAAGAA |
| Hes1 | CGCCGCCGCCGCCACTCG | AGCCTCCAAACACCTTAGCCGCCTCTCCA |
| Hey1 | TCGGCTCCTTCCACTTACTGTCTCCCAATG | AAGCTCCGATCTCCGTCCCCAAGGTCTAT |
| Gli1 | TGGTTCACATGCGCAGA | TTCCGGCACCCTTCAAA |
| Ptc1 | CACAGCCCCCAACAAAA | TGCCACCCACAATCAACTC |
| 18S rRNA | AGGACCGCGGTTCTATTTTGTTGG | CCCCCGGCCGTCCCTCTTA |
