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Representative X-ray images of harvested disks 4 weeks after the injection. (A) Control group: the disk received an injection of culture medium. (B) Immediate group: a cell sheet was injected immediately after implantation of the disk. (C) 1-wk group: a cell sheet was injected 1 week after the implantation. No obvious calcification was observed in the control group. The immediate group and the 1-wk group exhibited calcification around the disks. Arrows indicate areas of calcification.
