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. 2014 May 14;4:4955. doi: 10.1038/srep04955

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Passage of fluorescent dyes through (A) Panx1 and (B) Panx3 GJs. Dye was injected in one cell of a pair (donor cell; Cell-1) through the patch pipette and its passage to the recipient cell (Cell-2) was monitored. In 5 minutes time, anionic Alxa 350 reached to the recipient cell both through Panx1 and Panx3 GJs. None of the cationic dyes (e.g. PI, EtBr and DAPI) passed through Panx1 and Panx3 GJs, indicating the cells are not connected by cytoplasmic bridges. Dyes were not detected in the recipient cells even after 10 minutes. Cell-cell coupling was confirmed in every cell pair by measuring G_j at the end of the experiment. (C) As a positive control, Cx43-GJs expressed in HeLa cells showed the permeation of both anionic and cationic dyes. ‘BF' represents bright field image.