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. 2014 Mar 27;12:25. doi: 10.1186/1741-7007-12-25

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Double immunostaining for Isl1 and α-smooth muscle actin in mouse smooth muscle cells of the dorsal pylorus. Isl1 and α-SMA co-expression in smooth muscle cells at E11.5, E14.5, and E18.5. Yellow dotted lines mark the epithelial basement membrane, white thick dotted lines indicate ICM and OLM boundary, and white dotted lines indicate OLM and pancreas boundary. Red staining is Isl1, green staining is α-SMA, and DAPI nuclear counterstaining (DNA) is blue. α-SMA, α-smooth muscle actin; ICM, inner circular muscle; OLM, outer longitudinal muscle; Pa, pancreas; St, stomach. Scale bars: 50 μm.