Skip to main content
. 2012 Dec;53(3-4):240–252. doi: 10.1093/ilar.53.3-4.240

Figure 3.

Figure 3

CpG methylation profiling of murine Sox4 upstream region. Six polymerase chain reaction amplicons (numbered 1-6) representing the Sox4 upstream region (relative to the ATG start site) were generated from genomic DNAs isolated from gestational days (GDs) 12 to 14 secondary palate for CpG methylation profiling. CpG profiling was undertaken by amplifying bisulfite-modified DNA (Clark et al. 1994) and sequencing the cloned amplicons. Methylation levels of each CpG residue were inferred by sequencing approximately 10 clones. Amplicons 1 and 2 are poorly methylated (0-1.5%) and amplicons 5 and 6 are heavily methylated (86-96%) on all three GDs. Amplicons 3 and 4 are differentially methylated from GD 12 to GD 13 (upper box) but not from GD 13 to GD 14 (lower box). Note that methylation decreases from GD 12 to GD 13 in amplicon 3 and increases from GD 13 to GD 14 in amplicon 4. Changes in methylation levels in amplicon 4 are consistent with Sox4 messenger RNA expression (see text). No significant changes were observed in methylation levels in amplicons 3 and 4 from GD 13 to GD 14.