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. 2014 May 1;7:274. doi: 10.1186/1756-0500-7-274

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Copyright © 2014 Matysik and Kraut; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sum projection of a single molecule image stack, with color coding for better visualization: brighter color indicates longer time spent by the particle in a certain area. The detected particle trajectory is overlaid on the image. It can be clearly seen that a particle is switching between two states: fast diffusion and slow diffusion. This behavior is typical for most of the observed molecules. Analyzing Particle Movement (APM_GUI) software was used to ensure that the particles remain in a confined region longer than particles undergoing normal Brownian diffusion would remain. Scale bar 1 μm.