Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Anal Biochem. 2014 Mar 14;454:44–52. doi: 10.1016/j.ab.2014.03.003

Fig.6.

Fig.6

Reduction of EDTA or Tris-borate concentrations alters the migration rates of supercoiled and linear plasmid DNAs. (A) The mobility of supercoiled plasmid DNA (SC) relative to linear DNA (LIN) seen in a conventional 1 x TBE gel (left panel) is progressively altered when 1 x TB, 0.5 x TB and 0.25 x TB are used. (B) Substitution of free acid EDTA for disodium EDTA in 1 x TBE and 0.5 x TBE buffers results in normal migration of the SC and LIN forms. (C) In contrast to Tris-borate buffers, removal of EDTA from 1 x TAE does not alter the relative migration rates of SC and LIN DNAs. Each gel contained 1% agarose and was run at 10 V/cm.