Table 2.
Peaks observed in MALDI-TOF MS profile spectra tentatively assigned to known neuropeptides by mass matching
| Obs. m/z (Calc. m/z ) | Precursor (UniProtKB Entry name) | Peptide name | Peptide sequence | Previous ref. |
|---|---|---|---|---|
| 915.399 (915.415) |
FMRF_DROME |
SDNFMRPamide (FMRFamide 9) |
R.SDNFMRFa.G |
[1,2,7,9,30] |
| 925.481 (925.490) |
ALLS_DROME |
Drostatin-3 (Ast-A3) |
R.SRPYSFGLa.G |
[1,2,7,9,18] |
|
936.463 (936.473) |
TACHY_DROME |
TK-3 (dTK-3, APTGFTGMRamide) |
R.APTGFTGMRa.G |
[1,2,18] |
|
942.584 (942.589) |
TACHY_DROME |
TK-2 (dTK-2, APLAFVGLRamide) |
K.APLAFVGLRa.G |
[1,2,18] |
| 953.529 (953.521) |
ALLS_DROME |
Drostatin-1 (Ast-A1) |
R.VERYAFGLa.G |
[9,18] |
| 961.486 (961.504) |
TACHY_DROME |
TK-5 (dTK-5, APNGFLGMRamide) |
R.APNGFLGMRa.G |
[1,2,18,19] |
|
982.622 (982.606) |
SNPF_DROME |
sNPF-3 (KPQRLRWamide) |
R.KPQRLRWa.G |
[18] |
| 985.592 (985.588) |
SNPF_DROME |
sNPF-4 (KPMRLRWamide) |
R.KPMRLRWa.G |
[18,19] |
|
996.556* (996.572*) |
SNPF_DROME |
RLRF peptide 2 |
R.SPSLRLRFa.G |
[1,2,9,18,19,30] |
| 1005.557 (1005.512) |
FMRF_DROME |
SAPQDFVRSamide (FMRFamide 12) |
R.SAPQDFVRSa.G |
[9,19,30] |
|
1015.603 (1015.605) |
CP2B_DROME |
CAP-2 (CAPA-2) |
K.ASGLVAFPRVa.G |
[1,2,7,9,30] |
| 1065.560 (1065.552) |
TACHY_DROME |
TK-1 (dTK-1, APTSSFIGMRamide) |
R.APTSSFIGMRa.G |
[1,2,9,18] |
| 1076.560 (1076.568) |
TACHY_DROME |
TK-4 (dTK-4, APVNSFVGMRamide) |
R.APVNSFVGMRa.G |
[1,2,9,18,19] |
|
1112.529 (1112.520) |
FMRF_DROME |
TPAEDFMRFamide (FMRFamide 7) |
R.TPAEDFMRFa.G |
[1,2,7,9,19,30] |
| 1157.554 (1157.549) |
CORZ_DROME |
CorazoninAA3-AA11 |
T.FQYSRGWTNa.G |
[1,2,30] |
|
1161.551 (1161.558) |
AKH_DROME |
AKH Peptide (+ C-term GK) |
C.pQLTFSPDWGK.R |
[9,19,30] |
|
1186.538 (1186.511) |
DSK_DROME |
Drosulfakinin-1 (DSK-1) |
R.FDDYGHMRFa.G |
[9,19] |
| 1253.616 (1253.617) |
MIP_DROME |
Drostatin-B5 (Ast-B4) |
R.DQWQKLHGGWa.G |
[1,2,7,18,19] |
|
1276.696 (1276.680) |
ALLS_DROME |
Drostatin-4 (Ast-A4) |
R.TTRPQPFNFGLa.G |
[1,2,7,9,18,19] |
|
1294.681 (1294.673) |
CP2B_DROME |
CAP-1 (CAPA-1) |
R.GANMGLYAFPRVa.G |
[1,2,7,9,30] |
| 1324.727 (1324.717) |
Q59E62_DROME |
IFa-2 |
A.YRKPPFNGSIFa.G |
[19] |
|
1329.786 (1329.787) |
SNPF_DROME |
RLRF peptide 1 |
K.AQRSPSLRLRFa.G |
[9,18,30] |
|
1369.644 (1369.629) |
CORZ_DROME |
Corazonin (Crz Peptide) |
G.pQTFQYSRGWTNa.G |
[1,2,7,9,19,30] |
|
1423.814 (1423.827) |
NPLP1_DROME |
NAP peptide (+ C-term K) [NPLP1 (APK)] |
R.SVAALAAQGLLNAPK.R |
[7,9,18,19] |
|
1452.744* (1452.736*) |
CP2B_DROME |
CAP-3AA2-AA15 |
T.GPSASSGLWFGPRLa.G |
[1,2,9,19,30] |
|
1603.841 (1603.835) |
MIP_DROME |
Drostatin-B3 (Ast-B3) |
R.RQAQGWNKFRGAWa.G |
[9,18,19] |
|
1658.677 (1658.665) |
DSK_DROME |
Drosulfakinin-2 (DSK-2) |
R.GGDDQFDDYGHMRFa.G |
[9] |
|
1741.940 (1741.962) |
LCK_DROME |
Leucokinin (DLK) |
R.NSVVLGKKQRFHSWGa.G |
[1,2,7,9,19,30] |
|
2009.982* (2009.973*) |
PDF_DROME |
Neuropeptide PDF |
R.NSELINSLLSLPKNMNDAa.G |
[19] |
| 2176.201 (2176.188) | CP2B_DROME | CAP Propeptide 3 | R.GDAELRKWAHLLALQQVLD.K | [30] |
Ion masses observed to be isotopically resolved in both replicate experiments are underlined and bolded. As in Table 1, these reported observed m/z’s are the average of the m/z’s observed between the two replicate MALDI-TOF MS profiling experiments described. Truncated peptides are denoted by superscripts showing amino acids present from the annotated peptide sequence as described in Table 1. Peptide sequences include pre- and post- cleavage residues separated from sequences by a period. *All observed m/z’s listed are predicted to be monoisotopic [M + H]+’s of listed neuropeptides based on the calculated monoisotopic [M + H]+ (shown in parentheses below observed m/z) with the exceptions of m/z of m/z 996.572 and 1452.736 are [M + Na]+’s and m/z 2009.982 is an [M + K]+. C-terminal amidation is denoted by “a”, N-terminal pyroglutamation is denoted by “p”. Abbreviations: Ast – allatostatin, AKH – adipokinetic hormone, CAP - Cardioacceleratory peptide, NPLP1 - Neuropeptide-like precursor 1, PDF - Pigment-dispersing factor, sNPF-Small neuropeptide F, TK - Tachykinin-related peptide.