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Cytokine production of each Treg subset. (A) Production of IL-17, IL-2, IFN-γ, and TNF-α by each Treg subset after stimulation with PMA + ionomycin, and percentage of cytokine-secreting cells in each Treg subset is shown. Data are representative of 9 separate experiments. (B) The histogram represents the cytokine expression profiles of each Treg subset. Statistical comparisons were performed using the Student’s t-test.
