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. 2014 Apr 1;7:47. doi: 10.1186/1754-6834-7-47

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Copyright © 2014 Ciesielski et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of delamination and void area from TEM micrographs. (a) TEM micrograph of a region of cell wall from the HS reactor sample. (b) A binary version of the same image that delineates delamination/pore areas and also shows how representative intramural ROIs were chosen. (c) Delamination/pore areas measured by image analysis and presented as a percent of the measured area. HS, horizontal screw; ROI, region of interest; SG, steam gun; TEM, transmission electron microscopy; ZC, ZipperClave®.