Table 1.

Haematological, functional, and oxidative/nitrosative stress and inflammatory markers according to iron diet and DOX treatment.

	Group
	A	B	C	D	E	F
Parameter
Iron diet	LID	LID	LID	LID	ND	ND
DOX dose (mg/kg BW)	3	4	5	0	4	0
Hb (g/dL)	8.9 ± 0.5	8.6 ± 0.4	8.3 ± 0.5	10.3 ± 0.5†	13.4 ± 0.7‡	17.1 ± 0.5*
TSAT (%)	13.8 ± 2.3	11.9 ± 9.0	10.9 ± 4.3	12.7 ± 2.0	36.4 ± 3.0‡	41.8 ± 4.1‡
LVDD (mm)	8.61 ± 0.12	8.70 ± 0.14||	9.02 ± 0.13*	8.30 ± 0.12	8.28 ± 0.14	7.80 ± 0.10*
LVSD (mm)	5.60 ± 0.11	6.00 ± 0.10||	6.34 ± 0.11*	5.10 ± 0.10	5.20 ± 0.11	4.50 ± 0.12*
FS (%)	34.9 ± 1.7	31.0 ± 1.3||	29.6 ± 1.4*	38.6 ± 1.4†	37.2 ± 1.6†	42.3 ± 1.9*
Caveolin-1 (%/area)	18.2 ± 2.0	15.6 ± 1.8||	14.9 ± 2.1||	23.1 ± 2.2†	24.7 ± 3.0†	32.3 ± 2.7*
Cu,Zn-SOD (U/mg protein)	33.5 ± 2.3	37.2 ± 2.6||	40.1 ± 2.8§	24.7 ± 1.9†	28.4 ± 2.1†	18.9 ± 1.5*
GPx (U/mg protein)	423.2 ± 22.7	460.5 ± 26.4||	488.4 ± 30.1§	376.9 ± 32.4†	389.4 ± 27.4†	264.3 ± 20.6*
MDA (nmol/mg protein)	3.4 ± 0.1	3.7 ± 0.2||	4.0 ± 0.2§	2.8 ± 0.3†	3.0 ± 0.2†	1.9 ± 0.2*
GSH : GSSG ratio	3.0 ± 0.2	2.7 ± 0.1||	2.3 ± 0.3§	4.0 ± 0.3†	3.8 ± 0.2†	4.7 ± 0.3*
Nitrotyrosine (%/area)	24.1 ± 3.2	28.9 ± 3.7||	34.8 ± 3.0§	14.9 ± 2.6†	16.7 ± 2.1†	12.2 ± 2.0*
TNF-α (%/area)	18.7 ± 2.0	19.2 ± 1.6	20.1 ± 2.3	14.8 ± 1.7†	15.1 ± 1.9†	12.1 ± 2.0*

Data presented as mean ± SD. LID: low iron diet; ND: normal diet; DOX: doxorubicin; Hb: hemoglobin; TSAT: transferrin saturation; LVDD: left ventricular end-diastolic diameter; LVSD: left ventricular end-systolic diameter; FS: fractional shortening; Cu,Zn-SOD: copper-zinc superoxide dismutase; GPx: glutathione peroxidase; MDA: malondialdehyde; GSH : GSSG: reduced glutathione : oxidised glutathione; TNF-α: tumour necrosis factor alpha.

*P < 0.05 versus all groups; ^† P < 0.05 versus A, B, and C; ^‡ P < 0.01 versus A, B, C, and D; ^§ P < 0.05 versus A and B; ^|| P < 0.05 versus A.