Table 1.

Primer sequences used for real-time RT-qPCR

Gene	Genebank ID	Amplicon size	Forward sequence (primer #)	Reverse sequence (primer #)	Efficiency (qPCR)
Hipox	NM_001032376	142 bp	GCAGCCCCAGCGTCGTGATT	CGAGCAAGCCGTTCAGTCCTGT	99
Ppia	NM_214353	133 bp	TGCAGACAAAGTTCCAAAGACAG	GCCACCAGTGCCATTATGG	97
Ccl5	NM_001129946	78 bp	GAAATGGGTGCGGGAGTACA	GTTTGCACGAGTTCAGGCTC	94
Cox2	NM_214321	165 bp	TAGGATTCAGGGCTTTCACTGGCT	TGTCAGCCGACAATGAGATGTGGA	105
Ifnβ	NM_001003923	150 bp	TGCAACCACCACAATTCCAGAAGG	TCTGCCCATCAAGTTCCACAAGGA	102
Il1a	NM_214029	152 bp	TGAAGATGGCCAAAGTCCCTGACCT	ATCCATGCCGTCCCCAGGAAGTG	93
Il6	NM_214399	105 bp	ACTCCCTCTCCACAAGCGCCTT	TGGCATCTTCTTCCAGGCGTCCC	97
Il8	NM_213867	80 bp	TGTGAGGCTGCAGTTCTGGCAAG	GGGTGGAAAGGTGTGGAATGCGT	95
Il12a	NM_213993	162 bp	CTGAAGGCCGTCAGCAACAC	AGCCAGGCAACTCTCATTCG	92
Ifnλ1	NM_001142837	135 bp	TGGCCTTAGAGGCTGAGCTA	CCCTGATGCAAGCCTGAAGT	91
Irf1	NM_001097413	113 bp	AATCCAGCCCTGATACCTTCTCT	GGCCTGTTCAATGTCCAAGTC	95
Irf7	NM_001097428	94 bp	CTGCGATGGCTGGATGAA	TAAAGATGCGCGAGTCGGA	90
Plau	NM_213945	143 bp	CTCCAAAGGCAGCCATGAAC	CACAGTGCTCCCCTTGGAAT	106
Tnf	NM_214022	112 bp	GCCACCACGCTCTTCTGCCTA	ACGATGATCTGAGTCCTTGGGCCA	91
Vcam1	NM_213891	126 bp	TCCACGCTGGTCATGAATCC	TGGGTCCTTGGGGAAAGAGTA	101