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Intrinsic subunit separation activity associated with eRF1:eRF3. (A) Kinetics of peptidyl-tRNA release stimulated by eRF1(AGQ):eRF3. Symbols: ■ Dom34:Hbs1; ▲, eRF1(AGQ)/eRF3, ▼, mock; ◆, eRF1(AGQ)/eRF3 (GDPNP). (B) Stimulation of subunit separation capacity of eRF1(AGQ) by eRF3. Symbols: ■, Dom34/Hbs1; ▲, eRF1(AGQ)/eRF3; ◆, eRF1(AGQ); ▼, eRF3; ●, mock. (C) Native gel analysis of the codon dependence (UAA vs. CAA) of eRF1(AGQ):eRF3 recycling activity.
