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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Mol Biochem Parasitol. 2014 Feb 15;193(1):33–44. doi: 10.1016/j.molbiopara.2014.01.003

Fig. 3. Anti-LMW-PTP rabbit sera immunoprecipitates native LMW-PTP from amebic lysate.

Fig. 3

Two rabbits (UVA417 and UVA418) were immunized with wild-type recombinant LMW-PTP (Cocalico Biologicals, Inc., Reamstown, PA, USA). Lysate from 6.75x106 non-transfected trophozoites was immunoprecipitated with either pre-immune or immune rabbit serum using Dynabeads Protein A as per the manufacturer's instructions (Invitrogen/Life Technologies, Grand Island, NY, USA). Antibody (pre-immune or immune sera) was not crosslinked to the beads. Immunoblots were probed with either pre-immune or immune serum from the rabbit that was not used for immunoprecipitation of that sample. 100 ng purified recombinant wild-type LMW-PTP protein was loaded as a positive control. IP = LMW-PTP immunoprecipitated from lysate; Recomb = recombinant wild-type LMW-PTP protein. Arrow: expected size of the LMW-PTP (17 kDa). (A) Pre-immune serum from rabbit UVA417 was used to immunoprecipitate the native LMW-PTP from lysate, and the immunoblot was probed with pre-immune serum from rabbit UVA418. (B) Immune serum from rabbit UVA417 was used to immunoprecipitate the native LMW-PTP from lysate, and the immunoblot was probed with immune serum from rabbit UVA418.