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. 2014 Mar 14;15(1):194. doi: 10.1186/1471-2164-15-194

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© Hasin et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparative growth analysis of the Ssa1-4 in response to SDS. Overnight culture was diluted in fresh YPD medium to an OD_600nm = 0.1 and then the cells were grown to an exponential phase to a density of 3 × 10⁶ cells/ml. Cells were then re-suspended in fresh medium to a density of 5 × 10⁶ cells/ml and transferred to a microtitre plate. Representative spots shown in the figure are a neat concentration from a 1 in 5 serial dilution series. The plates were incubated for 3 days at 30°C.