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. 2014 Jan 26;16(6):787–799. doi: 10.1093/neuonc/not244

Fig. 4.

Fig. 4.

Flow cytometric (FCM) profile of putative CSC markers and examination of self-renewal capacity. (A) Flow cytometric (FCM) analysis of putative CSC markers during serial in vivo sub-transplantation (passages I to V) using fluorochrome-conjugated antibodies specific to CD133, CD15, CD44, CD24, and CD117 in freshly isolated IC-2664PNET cells. Appropriate isotype set was used as a negative control. (B) Profiles of CD133/CD15 and CD44/CD24 double-staining in IC-2664PNET. (C) Representative images showing the formation of neurospheres from the 4 subpopulations of FACS-purified cells double-stained with antibodies against CD133 and CD15. (D) Quantitative comparison of neurosphere-forming capacity in FACS-purified subpopulations of CD133+ and/or CD15+ cells as compared with CD133−/CD15− cells through limited dilution (P < .05). (E) Log-rank analysis of animal survival in mice engrafted with 1000 FACS-purified dual- and mono-positive CD133 and CD15 cells as compared with dual-negative (CD133−/CD15−) tumor cells (P < .05).