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Summary of immunofluorescence staining in neurosphere cells during serial in vitro passages.
| Marker | Target proteins | BXD-2664PNET (passage) |
|||||
|---|---|---|---|---|---|---|---|
| p6 | p9 | p19 | p29 | p39 | p54 | ||
| OTX2 | Stem cell | +++4 | +++4 | +++4 | +++4 | +++4 | +++4 |
| Nestin | Stem cell | ++4 | ++4 | ++4 | ++4 | ++4 | ++4 |
| GFAP | Mature astrocyte | – | – | – | – | – | – |
| NeuN | Mature neurons | +1 | ++2 | ++1 | +3 | – | – |
| Tuj-1 | Immature neurons | +++4 | +++4 | +++1 | +++2 | +++3 | +++2 |
| O4 | Oligodendroglia cell | – | – | – | – | – | – |
| Vimentin | Neurofilament | ++1 | ++3 | ++3 | ++3 | +++4 | +++4 |
