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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Neurobiol Aging. 2012 Dec 27;34(6):1653–1661. doi: 10.1016/j.neurobiolaging.2012.11.024

Fig. 3.

Fig. 3

Quantitative polymerase chain reaction (qPCR) was undertaken in hippocampal tissue to validate the age- and Alzheimer’s disease (AD)-related changes derived from the gene chip studies for VAMP2 (synaptobrevin 2), SNAP25 (synaptosomal-associated protein-25), synaptophysin, GRIN1 (glutamate NMDA receptor 1), muscarinic cholinergic receptor 3 (CHARM3), serotonin receptor 2a (5HTR2a), and somatostatin (SST) using hippocampal tissue from 29 samples (young control, n = 9; aged control, n = 10; AD, n = 10). (A) qPCR data confirmed widespread downregulation of these synaptic genes in aging, with further downregulation in AD. Comparison of relative expression changes derived from qPCR and microarray analysis (B) for the same set of samples revealed a high correspondence between the 2 methods.