Table 1.

Sequence of primers and MB probe sequence used in developing MB rRT-PCR assay for the diagnosis of CCHF.

Primer/probe	Oligonucleotide sequence (5′-3′)	Nucleotide position (JX051650)β
MB Probe	6-FAM-CGCGATCATCTCATCTTTGTTGTTCACCTCGATCGCG-BHQ-1	71–93
Forward primer	AGTGTTCTCTTGAGTGCTA	32–50
Reverse primer	CCACAAGTCCATTTCCTT	120–137

6-FAM: 6-carboxyfluorescein.

BHQ-1: Black Hole Quencher-1.

Underlined bases are molecular beacon probe stem.

^βNucleotide position with reference to NCBI accession number JX051650.