Table 2.
Intra- and interassay variability for the MB rRT-PCR assay on the basis of Cp values. The limit of detection was determined to be 7.6 copies. The assay was able to generate reproducible results with CV of <5% for both intra- and interassay. The intra-assay variability for TaqMan rRT-PCR assay is also shown on the basis of Cp value. For TaqMan assay the limit of detection is 10-fold lower than MB assay.
| CCHFV IVT-RNA copies | MB rRT-PCR | TaqMan rRT-PCR | |||||
|---|---|---|---|---|---|---|---|
| Intra-assay | Interassay | Intra-assay | |||||
| Mean Cp | SD | CV (%) | CV (%) | Mean Cp | SD | CV (%) | |
| 7.6 × 109 | 7.14 | 0.12 | 1.68 | 2.44 | 9.8 | 0.35 | 3.59 |
| 7.6 × 108 | 9.85 | 0.03 | 0.30 | 3.42 | 13.57 | 0.49 | 3.63 |
| 7.6 × 107 | 13.46 | 0.08 | 0.59 | 3.02 | 18.03 | 1.08 | 5.97 |
| 7.6 × 106 | 16.83 | 0.05 | 0.30 | 3.19 | 22.07 | 0.93 | 4.20 |
| 7.6 × 105 | 20.03 | 0.24 | 1.20 | 3.71 | 25.63 | 0.25 | 0.99 |
| 7.6 × 104 | 23.86 | 0.05 | 0.21 | 2.83 | 29.51 | 0.43 | 1.46 |
| 7.6 × 103 | 27.51 | 0.21 | 0.76 | 2.53 | 31.67 | 0.29 | 0.91 |
| 7.6 × 102 | 30.17 | 0.28 | 0.93 | 2.95 | 34.51 | 1.64 | 4.75 |
| 7.6 × 101 | 33.3 | 0.77 | 2.31 | 2.34 | 32.36 | 5.85 | 18.09 |
| 7.6 | 37.04 | 0.86 | 2.32 | 0.46 | — | — | — |