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Testing of clinical samples for CCHFV genome using RT-PCR (nested PCR) and TaqMan rRT-PCR for validation of presently developed MB rRT-PCR assay.
| Location (districts) | Species | Type of sample | Nested RT-PCR | TaqMan rRT-PCR | MB rRT-PCR | Results |
|---|---|---|---|---|---|---|
| Ahmedabad Mehsana Kutch Dahod Silvassa |
Cattle Buffalo Sheep Goat |
Serum | 03 | 03 | 03 | Positive |
| Blood | 03 | 03 | 03 | |||
| Ticks | 03 | 03 | 03 | |||
| Serum | 178 | 178 | 178 | Negative | ||
| Blood | 12 | 12 | 12 | |||
| Ticks | 13 | 13 | 13 | |||
| Total | 212 | 212 | 212 | |||
