Quantitative in vitro multiplexing of 4 SERRS probes (10–100 pM; blue: IR-780, red: IR-792, green: IR-797, purple: IR-813). Spectral range of 200–1500 wavenumbers was used for analysis. An error (the average difference between measured fractions and the predetermined ratio) of 9.6±3.7 %. Data was collected under 785 nm excitation (200 mW, 500 ms, 10 averages).