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. 2014 Apr 27;2014:852163. doi: 10.1155/2014/852163

Figure 7.

Figure 7

((a)–(d)) Double immunolabeling with TUJ1 (to label neuronal profiles, red), and anti-CD68 (to reveal microglial cells, green) antibodies in ventral ((a), (c)) and dorsal ((b), (d)) horn of WT ((a), (b)) and end-stage SOD1G93A ((c), (d)) mice; L: lateral, M: medial. CD68-positive profiles were rarely detectable in WT samples, but they were present in either large or moderate quantities in the ventral and dorsal horn, respectively, of SOD1G93A samples. In SOD1G93A dorsal horns, it was possible to observe some infiltrating microglial cells adjacent to medially located (presumably proprioceptive) axon fascicles, entering the spinal cord (arrows in (d)). (e) Quantification of CD68-positive profiles in the spinal cord of WT and end-stage SOD1G93A mice. Bars represent mean ± SEM values for counts performed in 6–11 fields of 2 animals per experimental condition; ***P < 0.001 versus WT (one-way ANOVA, Bonferroni's post hoc test). Scale bar in (b) = 100 μm (also valid for (a), (c), and (d)).