Table 2.
Antibodies used for immunohistochemistry.
| Antigen | Immunogen | Manufacturer, species, and type | Dilution used |
|---|---|---|---|
| TRPV1 | Synthetic peptide, RASLDSEESESPPQENSC corresponding to aa 4–21 of rat TRPV1 |
Neuromics Rabbit Polyclonal |
1 : 6000 (ABC), 1 : 1000 (IF), |
| Synthetic peptide, CGSLKPEDAEVFKDSMVPGEK corresponding to C-terminal residues 816–838 of rat VR1 |
AbCam Rabbit Polyclonal |
1 : 600 (ABC), 1 : 100 (IF), 1 : 1000 (WB) | |
| Epitope mapping near N-terminus of VR1 of rat origin | SantaCruz Biotechnology Goat Polyclonal |
1 : 800 (ABC), 1 : 100 (IF) | |
|
| |||
| CGRP | Synthetic CGRP from rat | Chemicon Int. Rabbit Polyclonal |
1 : 1200 (ABC), 1 : 600 (IF) |
| Synthetic rat alpha-CGRP conjugated to bovine serum albumin using glutaraldehyde | Enzo Life Sciences Rabbit Polyclonal |
1 : 5000 (ABC), 1 : 2000 (IF) | |
| Rat alpha-CGRP peptide | AbCam Mouse Monoclonal |
1 : 500 (ABC), 1 : 100 (IF) | |
|
| |||
| SP | Synthetic peptide: CRPKPQQFFGLM, corresponding to amino acids 1–11 of rat substance P | AbCam Guinea pig Polyclonal |
1 : 1500 (ABC), 1 : 500 (IF) |
|
| |||
| GAPDH | GAPDH from rabbit muscle | Millipore Chemicon | 1 : 1000 (WB) |