Table 2.

Summary of the HGF promoter sequencing

Patient no.	SNP (NCBI dbSNP)	INDEL mutation	NCBI dbSNP	DATE length	Sequence region	HGF Serum levels
MM 4	−1652C/T	–	Rs3735520	27	−2920 – +120	High
MM 5	−2142C/A	–	Rs11763015	26	−2870 – +120	High
	−1652C/T		Rs3735520
MM 7	−2142C/A	–	Rs11763015	22	−2910 – -20	High
		−1652C/T	Rs3735520
MM 14	2309 T/A*	–	Rs149178895*	29	−2920 – +120	High
	−1652C/T		Rs3735520
MM 22	–	–		15	−2280 – +120	High
MM 24	–	–	Rs3735520	28	−2870 – +120	High
		−1652C/T
MM 29	−1652C/T	–	Rs3735520	27	−2870 – +120	High
MM 3	−1652C/T	–	Rs3735520	22	−2870 – +120	Low
MM 12	−2142C/A	–	Rs11763015	22	−2840 – -1710	Low
MM 16	−1652C/T	–	Rs3735520	23	−2810 – +120	Low
MM 17	−1652C/T	–	Rs3735520	26	−2730 – +120	Low
MM 23	−2142C/A	–	Rs11763015	25	−2870 – +120	Low
	−1652C/T		Rs3735520

Genomic DNA isolates from CD138⁺ cells were used to amplify segments of the HGF promoter. PCR products were pre-treated with exonuclease I and shrimp alkaline phosphatase and directly used for Sanger DNA sequencing. RefSeq gene NG_016274 was used as reference sequence. SNP and sequence region are relative to transcription start site.

SNP positions Rs3735520 and Rs11763015 are depicted relative to transcription start site (NM_000601.4:c).

*SNP position RS149178895 is depicted relative to gene region NG_016274.1.