Table 1.
Oligonucleotides for qPCR
| Name | Sequence (5′-3′) | Target gene |
|---|---|---|
|
AM008 F |
ATGCAGCAGCGTGGAGAAGCT |
AM008 |
|
AM008 R |
AAGCTGGTCCTGTGTGGTTGTTACGT |
|
|
AM009 F |
AAGGGACGGCGAAGTCACAGC |
AM009 |
|
AM009 R |
TACTTGGACCTCAGGGTACATTTGGCT |
|
|
AM360 F |
TTGACTTACTCGCTGGTATCGCCTACAA |
AM360 |
|
AM360 R |
AAGAGTACCCAAGTATGCCAAAACCCGA |
|
|
AM366 F |
TATGGCGAGGAAGGCGTTCAAAGTC |
AM366 |
|
AM366 R |
TGCGAAGCACCGTACATGACGATT |
|
|
AM779 F |
AGGACCACAACCCCATCATGTTTGT |
AM779 |
|
AM779 R |
TTCCCTAGAGCAGAGGTCTAGTGAGT |
|
|
Msp3 F1 (2281) |
AACCCAACTTTCAACGGTATCAAGGACCT |
msp3 |
|
Msp3 R1 (2528) |
ATCCCTACTTCAACCCTGGCTCCT |
|
|
Msp1b F (229) |
TACGAGAGCGTGGGACTACGTGCTA |
msp1b |
|
Msp1b R (439) |
AAGCTGCTGCCTTGCCAAATTCTTG |
|
|
New msp5 F |
AAGTTGTAAGTGAGGGCATAGCCTCC |
msp5 |
|
New msp5 R |
AACTTATCGGCATGGTCGCCTAGT |
|
|
Omp4 F |
TTCCAACACACAGGAGGTGACACAC |
omp4 |
|
Omp4 R |
TTCTCTGCACCATAGCCCGCAA |
|
|
Omp8 F |
TTGCCCGAGCACCGAGATTTCT |
omp8 |
|
Omp8 R |
ATGGCTTTGCGTCTCCGTTCAG |
|
|
virB10 F |
ATCGTGGACGTAAGGACATTCCCCA |
virB10 |
| virB10 R | TGACTGTGAGTTGGTCTAGGGTCATCC |