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. 2014 Apr 24;7:66. doi: 10.1186/1754-6834-7-66

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Copyright © 2014 Kunze et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic overview of the n-butanol degradation pathway in A. adeninivorans.Arxula is able to use n-butanol as the sole carbon and energy source, by converting it into the central metabolite acetyl-CoA by ß-oxidation, to finally generate succinate in the peroxisomes. A genome-mining approach led to the proposal of the pathway shown here. The figure shows the reversible (double headed arrow) and irreversible (single headed arrow) reactions catalyzed by the corresponding enzymes (rectangular square) and the cofactors (ATP/AMP, NAD+/NADH) necessary for n-butanol degradation. Black marked symbols indicate intermediates occurring several times in the pathway. AMP, Adenosine monophosphate; ATP, Adenosine triphosphate; CoA, coenzyme A; NAD, Nicotinamide adenine dinucleotide; PP, phosphate.