Table 1.

Purification of KSI–p53pAnt fused peptide and p53pAnt peptide, overexpressed in E. coli BL21(DE3)

Purification step	Total fused peptide (mg)	Total p53pAnt (mg)	Purity (%)	Yield (%)
Total protein extract	n.d	n.d	43*	100
Inclusion bodies	n.d	n.d	79*	-
First affinity chromatography	184**	43	90*	-
Precipitation recovery	184	43	-	100*
Thrombin cleavage	-	-	-	95*
Second affinity chromatography	-	34**	95	85*

Data per g of dry cell weight.

Experiments n.d., not determined.

*Determined by SDS-PAGE gels densitometry analysis.

**Determined by Bradford assay.