Table 2.

Antibodies used for immunofluorescent staining of cultured cells

Target	Antibody	Dilution
β-tubulin	Monoclonal anti-beta-tubuline-Cy3	1/500
	clone TUB 2.1 Sigma C4585
Mucin	Monoclonal anti-human gastric	1/200
	mucin 5 AC clone 45 M1 Sigma M5293
Z0-1	Purified mouse anti-human ZO-1	1/100
	clone 1/ZO-1 610966 BD Biosciences
Mouse IgG1	Goat anti-mouse IgG1 AF488 A21121	1/600
	Molecular Probes™ Invitrogen
Control	Mouse IgG1 negative control X0931 Dako	1/50