Table 3.
Primer sequences, annealing temperatures of primer sets (°C), expected PCR fragment sizes (bp) and accession numbers or references
| Primer name | Primers sequence | Annealing temperature (°C) | PCR product (bp) | Accession number or reference |
|---|---|---|---|---|
|
ACTB
|
CACGCCATCCTGCGTCTGGA AGCACCGTGTTGGCGTAGAG |
63 |
100 |
[24] |
|
B2MI
|
CAAGATAGTTAAGTGGGATCGAGAC TGGTAACATCAATACGATTTCTGA |
58 |
161 |
[24] |
|
GAPDH
|
CTTCACGACCATGGAGAAGG CCAAGCAGTTGGTGGTACAG |
63 |
170 |
AF017079 |
|
HMBS-2
|
AGGATGGGCAACTCTACCTG GATGGTGGCCTGCATAGTCT |
58 |
83 |
[24] |
|
HPRT-1
|
GGACTTGAATCATGTTTGTG CAGATGTTTCCAAACTCAAC |
60 |
91 |
[24] |
|
KRT 8 |
TGACCGACGAGATCAACTTC TGATGTTCCGGTTCATCTCC |
60 |
294 |
NM_001159615 |
|
MUC1 |
TAAAGAAGACGGGCTTCTGG CCGCTTTAAGCCGATCAAAC |
60 |
134 |
XM_001926883 |
|
MUC2 |
ACCCGCACTACGTCACCTTC GGCAGGACACCTGGTCATTG |
62 |
150 |
BX671371 |
|
MUC4 |
CTGCTCTTGGGCACTATATG CCTGTGACTGCAGAATCAAC |
60 |
133 |
DQ848681 |
|
OCLN |
CTACATAATGGGCGTCAACC GGGCTGCTCGTCATAAATAC |
60 |
298 |
NM_001163647 |
|
RPL-19
|
AACTCCCGTCAGCAGATCC AGTACCCTTCCGCTTACCG |
60 |
147 |
[25] |
|
SDHA
|
CTACAAGGGGCAGGTTCTGA AAGACAACGAGGTCCAGGAG |
58 |
141 |
[24] |
|
TBP-1
|
AACAGTTCAGTAGTTATGAGCCAGA AGATGTTCTCAAACGCTTCG |
60 |
153 |
[24] |
|
VIL1 |
AGAAGTGGACGGTGCCCAAC TCTCGCCGATGAGGTAGGTG |
64 |
273 |
XM_001925167 |
| ZO-1/TJP1 | GAGGGCATTTCCCACGTTTC GCTTTAGAGCCGAGTCCTTG | 62 | 256 | XM_003353439 |
Reference genes are underlined.