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. 2014 May 15;10(5):e1003625. doi: 10.1371/journal.pcbi.1003625

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© 2014 Menolascina et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In-vivo control experiment were carried out via a microfludics-based approach, featuring a computer implementation of the control algorithm and an inverted microscope to sense the fluorescence signal. The computer uses the images taken by the automated microscopy unit to quantify cells fluorescence () and compare it with the desired amount . Once the control algorithm has computed the control action on the basis of the result of the previous comparison (), it varies the height of two syringes filled with either Galactose or Glucose. Hydrostatic pressure generated by the relative difference in the heights of the two syringes drives the flow in the microfluidic device and determines the type of sugar cells will sense within the chamber. Images are sampled at intervals of 5 minutes and used by the control algorithm to close the control loop.

Inline graphic — In-vivo control experiment were carried out via a microfludics-based approach, featuring a computer implementation of the control algorithm and an inverted microscope to sense the fluorescence signal. The computer uses the images taken by the automated microscopy unit to quantify cells fluorescence () and compare it with the desired amount . Once the control algorithm has computed the control action on the basis of the result of the previous comparison (), it varies the height of two syringes filled with either Galactose or Glucose. Hydrostatic pressure generated by the relative difference in the heights of the two syringes drives the flow in the microfluidic device and determines the type of sugar cells will sense within the chamber. Images are sampled at intervals of 5 minutes and used by the control algorithm to close the control loop.