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. 2014 May 15;9(5):e97269. doi: 10.1371/journal.pone.0097269

Figure 5. Use of flow cytometry side scatter pulse width (SSC-W) to estimate bead diameter.

Figure 5

Mean SSC-W was estimated for a range of silica and polystyrene size marker beads. (a) Histogram plots of silica beads (Si2: 2 µm; Si8: 8 µm) and polystyrene beads (P2: 2 µm; P7: 7.4 µm) contrasted with the SSC-W profile of Chlorella vulgaris (Cv). (b) Calibration curves of SSC-W vs bead diameter for silica (•) and polystyrene (▴) beads.